Complementary of water

COMPLEMENTARY COHERENT WATER

 

V.G. Krasnobryzhev

 

In recent years, the medical statistics indicates a deterioration of population’s health. The high anxiety is caused by the fact that only 20% of children are healthy. Thus, the perspectives for future generations in our country give rise to the preoccupation and the alarm.

In this case, we observe the interconnection between the state of health and the qualitative composition and structural properties of drinking water.

Undoubtedly, the state of our health is connected with the quality of drinking water. Indeed, 70% of an adult and 80-85% of a child consist of water. It is worth noting that water in human organism possesses specific properties such as the presence of some structure and a negative redox potential, which significantly influence the intracellular metabolism.

Many researchers assert that the usage of structurized high-quality water improves the health and affects the longevity of people. It is necessary to completely agree with this viewpoint.

However, these studies did not answer the following quite actual question: To which extent does drinking structurized water correspond to intracellular water of human organism? We know that intracellular water is also structurized and, in addition, its molecules have left torsion. Why? The answer is simple. The structure of all protein molecules of human organism is characterized by left torsion. Hence, drinking water must have also a structure with left torsion. However, the structure of water is represented by R- and L-tetramers in identical concentrations. Therefore, only a half of used water with left torsion enters the cells of organism. Water with R-torsion remains in the intercellular region, since it is not complementary to the protein-based structure of the membranes of cells.

In health organism, all processes run synchronously. In this case on the cellular level, the cells perform the informational exchange in the ultraviolet range with wavelengths of 240-380 nm. Despite a significant width of this range, the exchange is realized with the same phase, i.e., coherently. This yields the coherence and, respectively, the synchronism of all processes in human organism.

At a disease, the synchronism of the processes in organism is broken. This happens not only on the cellular level, but influences the endocrine, immune, and other controlling systems. But if a disease acquires the chronic course, the degree of desynchronization of organism increases, by causing the extension of a disease onto other organs and systems.

The synchronism of the processes in organism is also violated in the process of ageing. The authors of a lot of works indicate the development of dehydration of ageing organisms. In this case, there occur the slagging of cells, the desynchronization of intracellular and exchange processes, the catastrophic drop of the number of stem cells, which are suppliers of young cells, the deterioration of protective properties of organism, etc.

The answer to the question “What shall we do?” is unique. It is necessary to drink complementary coherent water, which differs from ordinary one only by that the structure of the former possesses the L-torsion, and its molecules oscillate with identical frequency and phase, i.e., synchronously. In this case, one says that each molecule feels all molecules of its environment.

It is significant that, under the recovery of the synchronous state of cellular structures, we may expect the intensification of the intracellular informational exchange and biochemical reactions, increasing the selectivity of processes, self-purification of surfaces from catalytic slags, etc. Therefore, by using complementary coherent water, we return the synchronism and the vital force to our organism and, hence, make it more healthy.

 

Complementary coherent water can be produced with the help of a special unit “Synchronizer.”

“Synchronizer” is a spintronic device possessing a large spin potential, which is conserved for 1.5 yr. It can be glued to the wall (or the bottom) of a ceramic mug or a glass 250–300 ml in volume. If we fill up it with water, then water becomes coherent in 5 min. We recommend you to wait 1 h and then to pour off water. You now have a simple system for the production of complementary coherent water. In what follows, you can fill up the mug with water, and it will become complementary coherent in 5 min.

Scheme of the usage of water.

  1. Complementary coherent water should be drink in the morning or in the evening prior to a sleep every day. The usage of water can be realized by the following scheme: in Your mug with “Synchronizer,” you fill up a portion of water 200-250 ml in volume and wait 5 min. Then, by holding the mug in your hand, you have to drink complementary coherent water during at least 5 min by small swallows. Due to the drinking of such water, the water medium of Your organism passes to the synchronized state, which causes the synchronization of all processes.

You should not expect the positive effect at once and forever. Miracles are met rarely and mainly in fairy tales. But the synchronism is a foundation of of the health of Your organism, which should be continuously supported.

Complementary coherent water will help You to counteract a wide spectrum of viral infections, which include, in the first term, herpes, vesicular stomatitis, HIV, hepatoinfections, etc.

The recovery of the synchronism of Your immunity will help You to resist the allergic diseases. Moreover, the synchronization of Your endocrine system will restore its regulatory functions. For example, You will be able to resist diabetes mellitus.

We do not promise the rejuvenation of Your organism without injection of stem cells, but Your health will become stronger.

 

Studies of the biological effiuciency of complementary coherent water

 

The ideology of studies is related to the following premises.

  1. Diseases and the ageing of organism are conjugated with the desynchronization of intracellular processes.
  2. The usage of complementary coherent water will initiate the synchronization of

intracellular processes, by supporting their intensity, will restore the correlations (interconnections and interrelations) between cells and their correlated behavior on the macrolevel, will improve the protective functions of organism, and will decelerate of the processes of ageing.

The studies of the efficiency of complementary coherent water were carried out by means of the determination of its influence on the operation of the immune system of infected organisms in vitro and in vivo.

The studies were performed at the Institute of Epidemiology and Infectious Diseases of the Academy of Medical Sciences.

                                      

  1. Cytologic and cytogenetic investigation of complementary coherent water

 

The culture of cells was obtained from 8-9-week human embryos by the standard procedure of trypsinization of disintegrated pieces. Cells were grown in nutrient medium RPMI-1640+10% embryonic calf serum + antibiotics.

For the cytologic studies, cells were grown on cover glasses in ordinary bacteriological test tubes. Cells were treated by a fixative, dehydrated, and colored with hematoxylin-eosin. We determined the following indices: proliferative activity (‰) and the presence of pathological mitoses.

Cytogenetic preparations were produced by the method given in “Method of human chromosome aberration analysis,” edited by K. Backton and H. Evans (WHO, Geneva, 1976). While studying the metaphases, we counted the number of chromosomes and aberrations in them.

To study the influence of complementary coherent water on the cytologic and cytogenetic indices, we treated cHET cells in the phase of logarithmic growth by complementary coherent water and produced preparations in 24 h. The control group included cells, which were not undergone any actions.

 

Results and their discussion.

 

Results of cytologic studies of the preparations, which were obtained on cHET cells and treated with coherent water, are shown in Table 1.

 

Influence of complementary coherent water on the mitotic mode of cHET cells

 

Table 1

Influence Мі (in ‰) Normal, in mitosis, ‰ Abnormal, mitosis, ‰
Cells +coherent water 21,0 91,4 8,6
The control of cells 29,0 93,2 6,8

 

Table 1 indicates that complementary coherent water rendered no significant action on the mitotic mode of cHET cells, as compared with cells in control.

In Table 2, we show the results of cytogenetic studies of cHET cells processed with coherent water.

 

Cytogenetic indices of cHET cells processed with complementary coherent water

 

Table 2

 

Influence

Influence Cells with number of chromosomes (in %)
45 46 47
The control of cells 4,0 96,0 0
Cells +coherent water 2,0 96,0 2,0

 

The data presented in Table 2 testify that the action of complementary coherent water rendered no significant action on the karyotype of cells and induced no aberrations (breaks) of chromosomes.

 

  1. Study of the interferonogenic activity of complementary coherent water

 

The interferonogenic activity of complementary coherent water was studied in experiments in vitro. For this purpose, we used leukocytes of donors. Into their 3-ml solution, we added 0.1 ml of coherent water, incubated the mixture at и 37оС for 18 h. Then we determined the activity of interferon in the supernatant fluid by the method of suppression of the cytopathogenous action of vesicular stomatitis virus in the homologous intertwined culture of cells L41 (human lymphoblast cells). The culture was grown in the medium consisting of nutrient medium RPMI-1640 + 10 % fetal serum + antibiotics.

To the monolayer of cells grown in dishes, we added the dilution of a cultural fluid containing complementary coherent water (after the incubation with leukocytes) and incubated. Then we remove the supernatant fluid and introduced vesicular stomatitis virus (VSV) in a dose of 100 TCD 50/0.1 ml. As control, we took the cultures of cells treated with VSV and cells undergone no treatment. Experimental and control cultures were incubated at 37оС for 24 and 72 h.

The determination of the activity of interferon was made in 24 and 72 h, when the introduced VSV caused the total degeneration of cells in control, whereas the intact culture of cells and the cells + complementary coherent water were not degenerated. As the titer of interferon, we took the quantity reciprocal to the dilution of coherent water, at which the culture in 50% of small cavities was completely protected from the cytopathogenous action of VSV.

The results of studies of the interferonogenous activity of complementary coherent water are given in Table 3.

 

Interferonogenous activity of complementary coherent water

 

Table 3

 

Influence

Interferon activity, units/ml
24 hour 72 hour
Spontaneous effect 4 4
The control of cells 128
Cells +coherent water 168 360

 

 

Thus, complementary coherent water activates the production of interferon and protects the culture of cells of leukocytes from the destruction.

           

  1. Study of the influence of complementary coherent water on the

           reproduction of vesicular stomatitis virus (VSV).

 

The study of the influence of complementary coherent water on the reproduction of VSV was carried out in the intertwined culture of cells L41 (human lymphoblast cells). Cells were grown in medium RPMI-1640 + 10% fetal serum + antibiotics.

Vesicular stomatitis virus, Indiana strain, was obtained from the Museum of viruses at the D.I. Ivanovskii Institute of Virology (RAMS, Moscow). The infection titer of the culture of cells L41 was 4.0-4.5 lg ID50. A monolayer of cells L-41 was grown in test tubes. Then VSV was introduced into test tubes in a dose of 100 ID50, and cells were treated by complementary coherent water. As control, we took the cultures of cells treated only with VSV and cells undergone no treatment. The infection titer of VSV was determined in the cultural experimental and control media after the 24-h cultivation at 37°С, when the full degeneration of the monolayer of cells in test tubes with VSV was observed. The results of studies of the influence of complementary coherent water on the reproduction of VSV are given in Table 4.

 

Influence of complementary coherent water on the reproduction of VSV

 

Table 4

 

Preparation

Credit of a virus in   lg ID50 Inhibition VVS in lgID50  

Р

Coherent water 3,0 3,0 <0,001
The control of cells 5,0

 

Thus, the studies performed indicate that, in the presence of complementary coherent water, the inhibition of the reproduction of vesicular stomatitis virus is equal to 2 lg ID50.

 

  1. Study of the anti-HIV activity of complementary coherent water

 

The study of the influence of complementary coherent water on the reproduction of HIV was performed on a new model of HIV-infection in COS cells, which possess the universal sensitivity to RNA- and DNA-containing viruses, including HIV.

The monolayer of cells COS grown in dishes was infected by HIV in a dose of 100 ID50 and cultivated for 5-7 days. We took the cultural fluid and determined р24 with the help of test-system Virognostica Organon in it. Then, by using 10-fold dilutions, we infected the culture of COS cells to determine the infectious titer. The results are presented in Table 5.

 

Characteristic of HIV-infection

 

Table 5

Processing of culture Days cultivation Level expression р24 HIV
р24, ng/mL infectious titer in lg ID50
HIV 5 3,640 5,0
HIV 7 3,666 6,0

 

The СОS model was used for the study of the influence of complementary coherent water on the reproduction of HIV. For this purpose, the monolayer of cells СОS was infected by HIV in a dose of 100 ID50 and treated with coherent water by the scheme used in the previous studies. We incubated cells at 37°С for 7 days and then determined the expression of р24 of HIV and its infectious titer in samples.

 

The results of studies of the influence of complementary coherent water on the reproduction of HIV are presented in Table 6.

 

Influence of complementary coherent water on the reproduction of HIV

 

Table 6

Preparations Expration, pg/ml Infectious titre of a HIV in lg ID50
HIV+coherent water 630 4,0
The control of a HIV 650 5,6

 

As a result of studies, we have established that complementary coherent water inhibits the reproduction of HIV by 1.6 1g ID50, which was revealed in the inhibition of the expression of antigene р24 of HIV-1 and in a decrease of the infectious titer by 1.6 1g ID50.

 

5. Study of the antiherpetic activity of complementary coherent water

 

To study the antiherpetic action of complementary coherent water, we used the model of herpetic meningoencephalitis. The given model is convenient for the estimation of the expression of symptoms, is characterized by the 100-% reproducibility, and requires no additional control. The development of clinic symptoms of the disease began in 5-6 days starting from the time moment of infection, attained the maximum in 13-14 days, and then was characterized by a decrease of the expression of symptoms with the subsequent recovery of survived animals. The presence of acute herpetic infection was corroborated by the method of immunofluorescence. For example, the most intense fluorescence was observed in tissues of brain (especially in trunk parts). It appeared in 6-7 days starting from the time moment of infection, which corresponds to the appearance of clinic signs of the disease. The less intense fluorescence (+ or ++) was observed in spleen and was absent in liver and lungs. Lethality of animals infected by herpes virus was 100%.

To model the experimental herpetic infection, we used simple 1-kind herpes virus. At the laboratory, virus made 28 passages through brain of white white. Prior to the experiment, virus was conserved in a 50% solution of glycerin in a phosphate buffer solution at a temperature of 10-15°С .

In experiments, we used white mongrel white 14-16 g in weight. The virus-containing material 0.03 ml in volume was introduced in mouse’s brain. The magnitude of infecting dose in experiments was equal to 1-10 ID50 (lethal doses for mice).

Complementary coherent water was introduced one time in the amount of 0.2 ml intraperitoneally by the following schemes: before the infection — prophylactic scheme of introduction; in 24 h after the infection by herpes virus — therapeutic scheme.

Each experiment included 2 groups of mice:

1 – mice under the action of complementary coherent water + herpes virus,

2 – mice, to which a physiologic salt solution + herpes virus were introduced.

The estimation of the activity of complementary coherent water was executed by comparing the lethalities of mice in experimental and control groups. In this case, we took into account

—  the lethality of animals (%),

— protection ratio (PR) – the ratio of the number of died mice in the experimental group to that in the control group,

— efficiency index (EI) of the preparation was calculated by the formula

EI        protection ratio

On the first stage of studies, we investigated the activity of complementary coherent water. To the animals infected by herpes, we introduced coherent water every 24 h up to the end of the experiment. The results of studies of the antiherpetic activity of complementary coherent water under the prophylactic action on mice are given in Table 7.

 

Antiherpetic activity of complementary coherent water

the anti herpetic activity of coherent water

 

Table 7

 

Preparation

Quantity

of mice

From them were lost Frequency rate of protection Index of efficiency
In total Percent
Coherent water 12 6 50 2,0 50,0
Placebo 14 14 100

 

As a result of studies, we have established that complementary coherent water activates the protective functions of the immune system against the herpetic infection.

 

  1. Pilot experiment on the inhibition of HIV in vivo at the usage of

    complementary coherent water

 

The experiment was executed in the SAR with patients (volunteers) infected by HIV with various contents of Т-lymphocytes CD4+ in blood. In the experiment involved  V.G. Krasnobryzhev,               S.V. Brukovsky, J.M. Kearney, E. Kearney

In the SAR, HIV-infected persons are divided into the following groups:

— group of patients ill with AIDS if the content of lymphocytes CD4+ <  200 un./µl in blood,

— group of HIV-carriers if the content of lymphocytes CD4+ > 200 un./µl in blood.

One part of patients used only complementary coherent water, whereas another part did complementary coherent water and antiretroviral drugs (ARVD). The results are presented in Table 8.

 

Influence of complementary coherent water on the content

of lymphocytes CD4+ in blood of HIV-infected patients

 

Table 8

 

The patient

 

Accepted preparations

Time of reception, days  

Contents CD4 + in blood

Water АRVM Before experiment After experiment
1/mcl % 1/mcl %
Michael Makhoba + 10 20 3,2 207 9,06
Rachel Ngcayiya + 10 90 6,96 154 11,05
Mercia Dube + 10 419 21,0 597 21,65
Petrus Tshabalala + + 30 101 6,68 156 7,54
John Moroazwi + + 30 271 18,8 453 21,25
Alfred Zulu + + 60 448 20,19 642 20,77

 

As a result of the executed experiment on the inhibition of HIV in vivo, we have established that complementary coherent water inhibits the reproduction of HIV, which causes the increase of the content of lymphocytes CD4+ in blood of patients. In addition, already during 10 first days, we observed the purification of skin of patients from eczematous and herpetic exhibitions, enlightening of urine, improvement of a sleep, and an increase of body’s weight.

 

Prof. Viktor Krasnobryzhev,

Kiev,

e-mail: vkentron@gmail.com

Tel. +380975609593